Executive Summary D 3.4.17_10.04.12

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Introduction Monitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. The Fluorescence in situ Hybridisation (FISH) technology has been proven to represent a sensitive and rapid molecular method for the specific detection of microorganisms. Within the TECHNEAU project a protocol for the simultaneous detection of E. coli and coliform bacteria based on the FISH technology was developed (Deliverables 3.4.1 and 3.4.15). Moreover, FISH-protocols for the detection and quantification of Pseudomonas aeruginosa (Deliverable 3.4.14) and Legionella spp. (Deliverable 3.4.16) have also been elaborated. The developed FISH protocols consist of two different approaches. One approach allows the direct detection of single bacterial cells on filter membranes. The second approach combines an incubation of the filter membranes on a nutrient agar plate and the subsequent detection of the grown micro-colonies.

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تاریخ انتشار 2010